|
|
|
| |
Results of Research
| TITLE: |
Tumor-specific
cytocidal and immunopotentiating effects of relatively low molecular weight products
derived from the basidiomycete, Agaricus blazei Murill. |
| AUTHORS: |
Fujimiya
Y; Suzuki Y; Katakura R; Ebina T |
| AUTHOR
AFFILIATION: |
Division
of Immunology, Miyagi Cancer Center Research Institute, Natori, Japan. |
| SOURCE: |
Anticancer
Res 1999 Jan-Feb;19(1A):113-8 |
| CITATION
IDS: |
PMID:
10226531 UI: 99243118 |
| ABSTRACT: |
Currently,
some natural herbal extracts are believed to have a marked tumoricidal effect and low
toxicity for normal tissues. We investigated the effect of relatively low molecular weight
products extracted from the basidiomycete, Agaricus blazei Murill, on MethA tumor cell
growth with the aim of producing synthetic derivatives based on these products.
Inoculation of the low molecule fraction (LM) into the primary tumor of a two-tumor model
resulted in the marked inhibition of the tumor, not only in the right flank, but also in
the non-injected left flank. Chromatographic purification and physicochemical
characterization showed the main tumoricidal activity to be located in a low molecule
fraction-3 (LM-3), containing alpha-1,4-glucan-beta-1,6- glucan complex with an average
molecular weight of 20 kDa. A11 LM fractions and crude ATF showed in vitro selective
cytotoxicity for MethA tumor cells, having no effect on normal cells. Serum levels of
immunosuppressive acidic protein (IAP) in mice receiving LM fractions, particularly LM-3,
significantly increased, indicating the possible activation of granulocytes. We speculate
that the inhibition of the distant tumor might be due to the increased migration of
granulocytes, enhanced by the effect of extract injections at the primary tumor site. |
| MAIN
MESH HEADINGS: |
Adjuvants,
Immunologic/*pharmacology
Agaricus/*chemistry
Antineoplastic Agents/*pharmacology
Glucans/*pharmacology |
| ADDITIONAL
MESH HEADINGS: |
Animal
Male
Mice
Mice, Inbred BALB C
Molecular Weight
Neoplasms, Experimental/drug therapy
Tumor Cells, Cultured |
| PUBLICATION
TYPES: |
JOURNAL
ARTICLE |
| CAS
REGISTRY NUMBERS: |
0
(Adjuvants, Immunologic)
0 (Antineoplastic Agents)
0 (Glucans) |
| LANGUAGES: |
Eng |
|
|
|
|
